Dr. Calvin A. Henard received a B.S. in Molecular Biology from Tarleton State University in Stephenville, Texas and a Ph.D. in Microbiology from the University of Colorado Anschutz Medical Campus in Aurora, Colorado where he studied the role of the nutrient starvation stringent response in Salmonella's ability to co-opt and subvert host immune responses. Following his doctoral studies, Dr. Henard was an NIH Ruth L. Kirschstein postdoctoral trainee at the University of Texas Medical Branch in Galveston, Texas where he evaluated virulence mechanisms of the intracellular protozoan parasite Leishmania. In 2014, Dr. Henard joined the National Renewable Energy Laboratory's National Bioenergy Center as a postdoctoral researcher, where he was promoted to a full-time staff researcher in the applied biology group in 2016. At NREL, Dr. Henard used his expertise in molecular microbiology and metabolic engineering to develop algal, yeast, and bacterial biocatalysts for conversion of renewable substrates to biofuels and bioproducts. In 2019, Dr. Henard joined the Department of Biological Sciences and the BioDiscovery Institute at the University of North Texas (UNT). Dr. Henard's lab at UNT leverages advanced molecular and synthetic biology to develop biotechnologies for the conversion of C1 substrates to fuels and chemicals using methanotrophic bacteria.
CURRENT RESEARCH INTERESTS:
- Genetic tool development
- Metabolic engineering
- Protein engineering
- Metabolic flux analysis
- Fermentation optimization
- C1 metabolism in methylotrophic bacteria
- Methylotroph physiology
- Bacterial stress responses
- Prokaryotic transcriptional regulation
- Redox signaling
MOST SIGNIFICANT PUBLICATIONS:
- Tapscott T., Guarnieri M.T., and Henard C.A. Development of a CRISPR/Cas9 system for Methylococcus capsulatus in vivo gene editing. Appl. Environ. Microbiol., 00340-19
- Fitzsimmons L., Porwollik S., Liu L., Chakraborty S., Desai P., Tapscott T., Henard C.A., McClelland M., and Vazquez-Torres A. Zinc-dependent substrate-level phosphorylation powers Salmonella growth under nitrosative stress of the innate host response. PLoS pathogens 14 (10), e1007388
- Qian F., Zhu C., Knipe J., Ruelas S., Stolaroff J.K., Deotte J.R., Duoss E.B., Spadaccini C.M., Henard C.A., Guarnieri M.T., and Baker S.E. Direct Writing of Tunable Living Inks for Bioprocess Intensification. Advanced Materials, Nano letters, 2019.
- Freed E., Fenster J., Smolinski S., Henard C.A., Gill R., and Eckert C.A. Building a Genome Engineering Toolbox in Non-Model Prokaryotic Microbes. Biotechnology and Bioengineering, 2018;1-19.
- Henard C.A. and Guarnieri M.T. 2018. Metabolic Engineering in Methanotrophic Bacteria. In Methane Biocatalysis:Paving the Way to Sustainability. M. Kalyuzhnaya and H.H. Xing, Eds. Springer Publishing.
- Henard C.A., Franklin T.G., Youhenna B., But S., Alexander D., Kalyuzhnaya M.G., and Guarnieri M.T. Biogas Biocatalysis: methanotrophic bacterial cultivation, metabolite profiling, and bioconversion to lactic acid. Frontiers in Microbiology, 9.
- Shaw J.A., Henard C.A., Liu L., Dieckman L.M., Vazquez-Torres A. and Bourret T.J. Salmonella enterica serovar Typhimurium has three transketolase enzymes contributing to the pentose phosphate pathway. J. Biol. Chem. 293(29):11271-11282.
- Akberdin I., Thompson M., Hamilton R., Desai N., Alexander D., Henard C.A., Guarnieri M, and Kalyuzhnaya M. Methane utilization in Methylomicrobium alcaliphilum 20Z R: a systems approach. Scientific reports 8, Article number: 2512 (2018)
- Henard, C.A., Smith, H., & Guarnieri, M.T. Phosphoketolase overexpression increases biomass and lipid yield from methane in an obligate methanotrophic biocatalyst. Metab Eng. 2017 May;41:152-158. doi: 10.1016/j.ymben.2017.03.007
- Henard, C.A., Smith, H., Dowe, N., Kalyuzhnaya, M. G., Pienkos, P. T., & Guarnieri, M. T. (2016). Bioconversion of methane to lactate by an obligate methanotrophic bacterium. Scientific reports, 6.
- "Organic Acid Synthesis from C1 Substrates," U.S. Patent Application 62/212,264 (2015)